P 3HB-co-4HB) was further P 3HB-co-4HB using methanol by droP 3HB-co-4HBd.
The supernatant was further precipitated with 4 volumes of ethanol to recover chondroitin and eventually residual low Mw HA.
With the removal of ethanol by rotary evaporation, Ats-loaded BSA NPs were further precipitated from the medium, and then 8% glutaraldehyde in water (0.5 μL/mg of BSA) was added to induce particle crosslinking under stirring of the suspension over a period of 24 h.
The eluted, DNase treated RNA was further precipitated by adding 1 µg/µl glycogen and finally dissolved in 10 µl of DEPC water.
DNA was further precipitated 30 minutes with isopropanol at -20°C.
DNA was further precipitated with alcohol in order to obtain highly purified DNA.
OMC lipoglycans extracted in the detergent phase were further precipitated by adding nine volumes of 95% cold ethanol at −20 °C for 12 h.
The BAC DNA was further precipitated at room temperature with 0.6 volumes of isopropanol for 30 min and centrifuged at maximum speed for 10 min. The pellet was washed with 70% ethanol, air dried shortly and dissolved in 25 μl of TE.
With longer periods of incubation, the precipitating concentrations of the same ions could be further reduced.
The salt-rich bottom phase was collected and further precipitated using ammonium sulphate at 60%% saturation.
