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However, the conjugative plasmids could not be further maintained in E.coli for more then a few generations.
This demonstrates that the conjugative plasmid can be conjugated to E.coli, but that the plasmid can not be further maintained.
In general, after the formation of hEBs in suspension for 2 4 days, they can be further maintained in culture for approximately one month with renewal of the medium every 2 3 days without passaging (Fig. 1Ai and ii).
Through examination of the financial maintenance achieved by some resource centers for sustaining their core activities and assessment of how these may be applied towards long term preservation of databases it has become evident that most databases obtain an initial funding for a particular project, and then need to be further maintained through institutional or government funds.
Similar(56)
They were cleaned with distilled water to remove the attached dirt and soil and were further maintained in tap water with added nutrients (potassium and nitrate salts) till further experimentation.
This perception is further maintained and strengthened by the Chinese clan system; elderly people are the center of families, and family ethics emphasizes benevolent fathers and filial children, with expectations of respect for elders and care for juniors.
This quality is further maintained by stringent protection of the watershed; boating and swimming are prohibited at Hetch Hetchy Reservoir (although fishing is permitted at the reservoir and in the rivers which feed it).
The cells were further maintained for 36 hrs after transfection.
The mutant line was further maintained by mating between siblings.
The level of DNA-PKcs expression was further maintained in the differentiated adipocyte (D7 and D14).
MCF-7 Tet-off-induced repression of ACSL4 cells, designated MCF-7 Tet-off/ACSL4, were further maintained in complete DMEM.
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