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3) The demonstration that monocytes do extravasate into the interstitial space needs to be further established using confocal analysis of lung sections stained with different endothelial markers.
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The targeting ability of PA-PEG1k-RGD was further established using a competition assay.
The psychometric properties of the finalised QUAD were further established using data from the MIRIAD study.
The link between miR-106b∼25 clusters and its gene target (HMGA2) was further established using the short antisense oligos (antagomirs) against the individual miRNAs of this family.
The absolute requirement of Ser was further established using co-IP of overexpressed dominant negative PDE4D3: while RXFP1, AKAP79, AC2-HA, β-arrestin 2 and PKA all associated with PDE4D3, these interactions were abolished if Ser was mutated.
The special role of spatial location in visual perception and selective attention has been further established using a range of different paradigms (e.g., Johnston & Pashler, 1990; Kwak & Egeth, 1992; Sagi & Julesz, 1985).
The role of NF- κB and ELK1 was further established using the NF- κB inhibitor (PDTC) and I κB protease inhibitor (TPCK) or ELK1 and p65 small interfering RNA (siRNA).
The anti-apoptotic role of cGMP/PKG signalling pathway has been further established using natriuretic peptides, atrial natriuretic peptide, and B-type/brain natriuretic peptide (BNP), which activate cGMP/PKG via an alternate pathway (i.e., activation of particulate guanylyl cyclase).
The physiological relevance of HF-related microRNAs on the expression of putative gene targets was further established by using gain-of-function assays in two human cardiac-derived cells.
The multiplex PCR assays were further established for the joint use of some SCAR and ITS markers efficiently.
Further characterization of breast CSCs was established using ALDH1 [ 79], mammosphere assay [ 80], and transplantation into immunodeficient mice [ 81].
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