Sentence examples for be further digested by from inspiring English sources

Nt or Nb.BspQI-nicked dsDNA can be further digested by E. coli exonuclease III to create ssDNA for downstream applications.

Then, the phosphorylated target DNA can be digested by λ exonuclease and the probe DNA was remained on the electrode surface, which can be further digested by exonuclease I.

Taken together, the in vitro reconstituted cleavage reactions demonstrate that 4B-dependent cleavage of 4FA generates a substrate that can be further digested by CtpB.

Proteasomal cleavage products (epitope precursors) might be further digested by cytosolic peptidases before the transporter-associated-with-antigen-processing (TAP) transfers them into the endoplasmatic reticulum (ER).

We inserted a ccdB fragment with corresponding BsmBI site into the assembled vector, named pLVX/U6-ccdB-CMV-AGO2, which can be further digested by BsmBI, leaving five overhangs of 5′-CGGT and 5′-TTTT for the ligation of an annealed shRNA insert with 5′ overhangs of 5′-ACCG and 5′-AAAA.

Afterwards, the digested glycopeptides were further digested by PNGase F (NEB) to remove the glycans attached to the proteins, and were labeled by H2O (Sigma) to confidently assign the N-glycosylation sites.

The recovered chymotryptic peptides were further digested by 1 µg of V8 protease for another 1 hr at 25°C.

Subsequently, pre-miRNAs are exported into the cytoplasm, and are further digested by Dicer, which is an endonuclease that cleaves double-strand RNA or pre-miRNAs, and become a short duplex RNA.

The pellet was further digested by a second treatment with trypsin for 20 min at room temperature, before centrifugation at 300 g 1 min. The supernatant was poured into 20 x volume of extraction media.

SMART-amplified cDNA samples were further digested by Rsa I endonuclease.

Tissue was further digested by addition of 4 μl Proteinase K (10 mg/ml) and incubation at 50°C overnight.