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The co-factors required for NaV1.9 expression have not been defined, but this channel can only be functionally expressed in dorsal root ganglion neurons where it rescues the expression of persistent current in NaV1.9 knockout neurons (Ostman et al., 2008).
However, it remains unclear whether mammalian DNMTs can be functionally expressed in yeasts, which probably lack endogenous DNMTs.
Our results indicate a new strategy for improving yeast-based production of nitrogen-containing chemicals and demonstrate that Ni2+-dependent enzymes can be functionally expressed in S. cerevisiae.
The murine single-chain variable fragment F8 (scFv(F8)) is endowed with high intrinsic thermodynamic stability and can be functionally expressed in the reducing environment of both prokaryotic and eukaryotic cytoplasm.
The cluster was highly unstable on a variety of shuttle plasmids but could finally be functionally expressed in its entirety in Streptomyces lividans using a novel integrative cosmid vector.
We report the design, construction and use of an antibody bacteriophage display library built on the scaffold of a single-chain variable fragment (scFv) previously proven to be functionally expressed in the reducing environment of both bacterial and plant cytoplasm and endowed with intrinsic high thermodynamic stability.
Similar(36)
To date, there are only a few members of the V1r and V2r families of receptors that have been functionally expressed in heterologous expression systems (Shirokova et al., 2008; Dey and Matsunami, 2011).
In this study, pullulanase gene (pulN) was functionally expressed in E. coli through expression vector replacement and induction expression strategy optimization.
This novel clone was functionally expressed in both prokaryotic and eukaryotic expression systems and exhibited ODD activity.
Several genes each over 10,000 base pairs in size had never before been functionally expressed in E. coli.
The CPPS and KS from S. rebaudiana were functionally expressed in a heterologous host E. coli.
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