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Both of these can be functionally evaluated in tomato using natural variation for drought adaptive traits.
3, 160 Finally, due to its accessibility, candidates can be functionally evaluated in developing retinas using in vivo electroporation to either drive overexpression or knockdown expression of candidate molecules.
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Two approaches were used: (i) CD133 expression was functionally evaluated in HT29 after gene knockdown and (ii) SW480 underwent cell sorting into a CD133+ population and a CD133− population followed by comparative functional analysis of the two populations.
Moreover, the purified rhANXA5 protein was functionally evaluated in a FITC-annexin V binding experiment and the results demonstrated that rhANXA5 detected apoptotic cells similarly to a commercial kit.
One clinically important, but poorly understood, phenomenon which could be functionally evaluated with this method is the development of disc hemorrhages in glaucoma.
or otherwise cannot be functionally evaluated were classified as uncharacterized RNA (Additional file 5: Table S4).
PBMC versus CD8-depleted (CD8D) CS, 20 kDa versus 5 kDa molecular weight cut-off (MWCO) bioreactor cartridges, 7- versus 10-day culture periods, and varying concentrations of IL-2, fetal bovine serum (FBS) and glucose content in the medium were functionally evaluated for p24 yield.
All three mice were functionally evaluated before and after starting the injection experiment (in the 4th week), and euthanized for molecular and histological studies.
The sorted populations were functionally evaluated by their ability to form colonies and mammospheres, in vitro.
At 48 hours after ischemia, animals were functionally evaluated using the Tarlov score.
All patients were functionally evaluated using Majeed's grading scale with mean follow-up time of 18.9 months (range 14 25 months).
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