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The effectiveness of our dUTPase complementation also suggests that this protein has no critical partners, the independent evolution of which would have precluded it to be functional in different backgrounds.
This means that a PCS should have a flexible design and be functional in different settings.
However, miRNAs of 23 nt were predominant in schistosomulum (Fig. 3b) indicating that several miRNA processing pathways might be functional in different developmental stages of the parasite.
Although proteins can be functional in different compartments (e.g. importins that shuttle other proteins into the nucleus), most proteins of known function complete their tasks as 'natives' of one particular compartment.
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For example, VAMP-3 can functionally replace VAMP-2 and several different SNARE complexes are functional in different cell compartments like ER, golgi, endosomes, plasma membrane, etc., It will be important to determine whether specific v-or t-SNAREs play a more important role in budding than others.
Each promoter is functional in different cell types and/or under different physiological conditions (Muhlethaler-Mottet et al, 1997; van der Stoep et al, 2002).
Thus, the expression of each gene appeared to be functional in a different space in hexokinase.
Second, whereas removal of some components may indeed render a biological system nonfunctional for its current role, it may still be functional in a different way.
This apparent problem can be explained by the fact that a third hormone, 11-deoxycorticosterone (DOC) can still bind; therefore, the receptor can still be functional (in a different manner) if the S106P mutation occurs first.
The cloning of 95% of the S. aureus ORFeome was achieved, and a random sampling of ORF-containing plasmid vectors showed the ORFs to be functional in two different downstream applications: recombinant protein production in E. coli and a protein interaction assay (Y2H).
I propose that the Drosophila method can be readily adapted in other organisms without culturable stem cells, since the mechanism for in vivo donor generation in Drosophila is likely to be functional in a variety of different organisms.
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