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The observation that several cultured cell lines and tissues express multiple isoforms of Abi1 [21] point to the possibility that multiple Wave 2 complexes, i.e., associated with different Abi1 isoforms, might be functional in cells.
Another near-IR probe uses DPA as a Zn2+-binding group and tricarbocyanine as a fluorophore and was also shown to be functional in cells.
Also, these methods may not be functional in cells that do not contain viral molecules, as quoted by Waheed et al (1999).
These signaling mechanisms are widely assumed to be functional in cells chronically exposed to TNF-α and to mediate the pathogenic effects of TNF-α in chronic inflammation.
Nevertheless, this suggests that such an inhibiting/attenuating mechanism can also be functional in cells which only express an FcεRI lacking the β-chain, such as Langerhans and dendritic cells [ 22].
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A genetically encoded variant of this sensor is shown to be functional in cell lysates and in mammalian cell cultures.
► Nitrate transporters are functional in cells lacking nitrate reductase.
The probe was functional in cells and could detect exogenously loaded Fe3+ in mitochondria.
This probe has nanomolar affinity for Zn2+, is functional in cells, and can be used in two-photon experiments.
SiR-Zn features nanomolar affinity for Zn2+, a 15-fold increase in fluorescence upon Zn2+ binding, and is functional in cells.
This notion is supported by the finding that Snc1 was retained in endosomes while Chs3 transport was functional in cells in which the ArfGAP1 GCS1 was deleted (Robinson et al., 2006).
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