These experiments show this protein is functional in binding both FITC or carboxyfluorescein on one end and MG-2p on the other end, leading to decreased green fluorescence and increased red fluorescence in a concentration-dependent manner.
While PEGylation does increase the thermal stability of ConA, the PEGylated protein must maintain its binding capability to be functional in a competitive binding assay.
However, both of the YF1 and YF2 mutants of v225d CagA retained the ability to interact with SHP2, ruling out the possibility of a cis-interaction while showing that each of the EPIYA-D/CΔCM and EPIYA-pD/CΔCM segments is functional in SHP2 binding.
Although silMUT contains the peptide G binding region and is functional in laminin-1 binding, it is missing portions or all of the other two laminin-binding sites.
The depleted vesicles, containing a full complement of SRP receptor but deficient in the glycoprotein subunit of signal peptidase, the ribophorins, and other glycoprotein components, were functional in the targeting and binding of nascent preprolactin but exhibited a greatly diminished capacity for translocation.
P. aeruginosa EF-Tu was active in forming a ternary complex with GTP and aminoacylated tRNA and was functional in poly(U -dependent binding of Phe-tRNAPhe at the A-site of P. aerU -dependentsomes.
Additionally, we confirm that newly identified low-affinity enhancer binding sites are functional in vivo, and that their contribution to gene expression matches their predicted affinity.
Overall, these results demonstrated that only the second miR-155 binding site is functional in KGF 3'-UTR.
DOI: http://dx.doi.org/10.7554/eLife.03613.017 Not all 68,000 detected HNF4A binding sites are functional in terms of gene regulation.
SiR-Zn features nanomolar affinity for Zn2+, a 15-fold increase in fluorescence upon Zn2+ binding, and is functional in cells.
