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HK proteins are evolutionarily conserved between plants and bacteria and plant HK components have been shown to be functional in bacteria.
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Because all modules of the AND gates are functional in bacteria other than Streptomyces, we anticipate that these regulatory devices can be used to control gene expression in other Actinobacteria.
The plastid P16S promoter has proven to be functional in multiple bacteria species [ 29].
In the case of the AcrIIA2 and AcrIIA4 synthesized constructs, the upstream region was replaced with that of AcrIIA5 to ensure a ribosome binding site known to be functional in lactic acid bacteria, and a single nucleotide substitution (C-A) was made to avoid the rare CGC arginine codon.
Therefore, these genes transferred from bacteria or fungi appeared to be functional in shrimp cells.
It should be functional in the next two weeks.
We did not attempt to determine whether LEE was functional in these bacteria.
They are functional in a restricted sense only.
Only the m2 mutant was tested and found to be functional for RMCE in bacteria and Cre-expressing eukaryotic cells (HEK-293).
Only eleven bacterial species possess all proteins essential for a functional PAR metabolism, although it is not known whether PAR metabolism is truly functional in bacteria.
Similarly, GlnRS present in lone members non-proteobacterial phyla (Additional file 2), like Acidimicrobidae bacterium (actinobacteria), Ignavibacterium album (green sulphur bacteria), Anaerolinea thermophila (green non-sulphur bacteria) or N. defluvii (hyperthermophilic bacteria) may not be functional.
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