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These three genes are unlikely to be functional due to the absence of the homeodomain indicating that these paralogues have become pseudogenes.
However, no native transposon has so far been recruited for mutagenesis in H. volcanii, and heterologous systems may not be functional due to the high (1 to 2 M) intracellular potassium chloride concentration in haloarchaea.
As mentioned before, Pho boxes are found for the pstC-A-B operon in CCMP1375 through phylogenetic footprinting, but they are unlikely to be functional due to the lack of sphR gene in the genome.
The intron 8 (c.775-41G>C) variant was not tested for in controls, but it is also unlikely to be functional due to its deep intronic location and the lack of predicted splicing effects.
Although wild-type B3GALT6 is expressed in the Golgi, the mutant enzyme (p.Met1?) is localized in the nucleus and cytoplasm [ 70], indicating that the mutant protein may not be functional due to its cellular mislocalization.
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DOI: http://dx.doi.org/10.7554/eLife.02025.012 It is conceivable that with a partial depletion of the factor required for cenH3-assembly into chromatin, relatively high cenH3 occupancy is maintained at the sites that are functional due to perdurance of the protein.
It follows that early signalling might be profoundly impaired but later steps may be still functional due to unknown adaptation processes.
Although HDA10 and HDA17 both contain the conserved RPD3 histone deacetylase domain, they may not be enzymatically functional due to the absence of an active site.
Even in vertebrates, DNA sequences conservation is low and suggests that most lncRNAs are not functional due to insufficient selection pressure.
all atoms in oxirane, aziridine and thiirane rings (such rings are traditionally considered to be functional groups due to their high reactivity).
While Wohlwend's design style is "simple and elegant", the game's minimalism was also functional due to his inexperience with programming.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com