sima null mutant individuals are unable to carry out transcriptional responses to hypoxia, although they are fully viable in normoxia.
In addition to the skin being fully viable and in its complete physiological environment, our study adds three important levels of complexity to current knowledge derived from previous in vitro experiments: (a) a fully functional circulatory system, (b) a fully functioning immune system, and (c) physiological flexing and movement of the skin.
The mutant mice were fully viable, fertile, apparently normal in appearance and behavior, and expression of other HIF-3 α splicing variants appeared not to be disturbed.
Consistently across the allelic series, our heterozygous Foxp2 mutants are fully viable and healthy, without delays in weight gain or in righting-reflex maturation.
In contrast, cells in which Bem1-CAAX replaced Bem1 were fully viable and grew well at all temperatures, so in most subsequent experiments we used Bem1-CAAX.
We have previously described that the Vglut2 f/f TH - Cre cKO mice are fully viable but that TH-Cre activity in the DRG leads to an itch phenotype in some Vglut2 f/f TH - Cre cKO mice at a late adult stage (Lagerström et al. 2010).
These time points were chosen to capture labile, cytokine-responsive transcripts within a temporal window in which cells were fully viable.
Since the Grp94+/− pups in our model were born in the expected Mendelian ratio of 1∶2 with respect to Grp94+/+ pups, our results suggest that the Grp94+/− embryos in our model were fully viable.
Surprisingly, this strain was fully viable, suggesting that the dissociation of Dre2 from Tah18 in wild-type cells might not be essential for viability.
In contrast, all Inv uvrD mutants were fully viable on LB as on MM.
