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To our knowledge Bn57 is the first CMV strain isolated from P. vulgaris to be fully sequenced and cloned, providing a useful tool for analyses of CMV-host interactions.
To achieve this goal, we developed a streamlined and easy-to-follow approach to the design, validation and annotation of species-specific array probes, particularly for organisms whose genomes have yet to be fully sequenced and annotated.
Despite the continuing development of these analytical methods, the reality of the situation is that only short oligosaccharides can be fully sequenced, and the prospect of unmasking the structure of intact HS chains remains a formidable challenge.
The genome of Sc. pombe, published in 2002, was the second unicellular eukaryotic genome to be fully sequenced and the sixth eukaryotic organism overall (Wood et al., 2002).
In the future, more embryophyte genomes will be fully sequenced and this will be reflected in the database that was used for this study.
Cichlid genomes have yet to be fully sequenced and annotated; therefore, we first annotated cichlid 3′-UTRs from partial genomic sequence.
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Only one chromosome, No. 22, has been fully sequenced and assembled, a task completed in December by the Sanger Centre of England and others.
Finally, the mtDNA of most common species have been fully sequenced and are freely available.
Saccharomyces cerevisiae is one of the best genetically characterized yeast as its genome is fully sequenced and analyzed exhaustively.
The genome of this phage has been fully sequenced and is composed of 52,141 bp of linear double stranded DNA with a GC content of 61.49%.
Twelve randomly selected clones were fully sequenced and showed 9 ± 2 crossovers and 1.5 ± 0.5 spontaneous mutations per ∼1.5 kbp open reading frame.
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