When these macrophages were fully differentiated and treated with the TLR2 ligands: LTA, R-FSL1, S-FSL1, Pam2CSK4, or Pam3CSK4, once again there were ligand-specific activation kinetics of the p38, SAPK/JNK, and NF-κB pathways, which precisely matched those observed using the immortalized Raw 264.7 cells (Fig. 2A C).
Therefore, though most of CD8+ T cells recovered after the transfer of CD8+ T cells alone were CD62Lhigh, the presence of CD4+ T cells promoted a massive increase in the accumulation of CD8+CD62Llow cells, which are fully differentiated and express more killer function molecules than memory cells or effector CD8 cells recovered at the peak of the primary immune response [28].
After 7 days in culture, the CGNs are fully differentiated and functional.
In adults, the pituitary is fully differentiated and contains all of the hormone-secreting cell lineages.
Like the primary leaves, these leaves are fully differentiated and characterized by multiple linear segments.
During this stage, the heart chambers are fully differentiated and all the major organ systems are developed and fully functional.
Immediately after birth, multiple mouse skeletal muscles contain both slow- and fast-twitch fibers; following postnatal development and growth, fiber type composition of skeletal muscles is fully differentiated and maintained throughout adulthood before aging.
108– 111 Remarkably, fibroblasts, though supposed to be fully differentiated cells, can be isolated and cultured in exactly the same conditions.
