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Callisto's interior is in stark contrast to that of Ganymede, which appears to be fully differentiated.
Ganymede appears to be fully differentiated, consisting of an iron sulfide iron core and a silicate mantle.
108– 111 Remarkably, fibroblasts, though supposed to be fully differentiated cells, can be isolated and cultured in exactly the same conditions.
In addition, viral infection and upper respiratory infection are separate ICD-9-CM codes, but likely represent a spectrum of overlapping diseases that cannot be fully differentiated using this database.
This is not surprising when considering that the pro-neurogenic effects mediated by ADs would not be of neurobiological significance at short-term, since a newborn neuron takes approximately a 5 weeks period to be fully differentiated and integrated in the neuronal circuitry of the adult DG.
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However, analysis by using anti-MyHC (myosin heavy chain) antibody indicated that the muscle cultures we established in our laboratory are fully differentiated after 5 days in differentiation medium [18].
What these three stages have in common is that they are fully differentiated cells that will undergo rapid cellular differentiation and/or growth expansion upon entering a new environment.
RT-PCR analysis was done on day 14 based on the fact that the mononucleated cell line (MC3T3-E1) is fully differentiated into osteoblast cells after 14 days in differentiation medium [ 14].
After 3 or 4 days in Differentiation Medium, when the C2 myotubes are fully differentiated, prepare 12mm glass coverslips coated with laminin for microscopy, or 100mm culture dishes coated with laminin for biochemical assays.
The cells started to differentiate into adipocytes as early as day 3 and were fully differentiated at day 14 as consistent with increased cell vacuolation of fatty acid observed under the light microscope using Oil O Red staining (Fig. 5a).
Unlike embryos, their tissues are fully differentiated.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com