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Samples were shipped by courier to the Paterson Institute for Cancer Research in Manchester in solid carbon dioxide, and confirmed to be frozen upon arrival.
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Suppressing my fear of being frozen upon entry, I opened the door and beheld a storeroom filled with boxes of expired vitamins from the "nutritional supplement" store upstairs.
He drinks healthy smoothies and takes vitamin injections, but he's been smoking for over forty years (perhaps he figures that, if he's frozen upon death like he plans, he can get his lungs cleaned up in the future).
All samples were frozen upon arrival to reduce the effect of potential degradation due to storage.
RT4-D6P2T cells were cultured for less than 2 months after reconstitution from stocks, which were frozen upon receipt from the ATCC.
Because the inocula had been frozen upon collection, 100-mL aliquots of each were defrosted at 30°C and then immediately placed under anaerobic conditions into sterile pre-reduced 500 mL Duran bottles.
But when that system abruptly collapsed in 1989, this problem, like many others to which Communism had promised definitive answers, turned out merely to have been frozen – and upon thawing it was in many ways worse than before.
Indeed, slow evaporation allows partial coalescence of the soft particles swollen by the organic solvent, whereas the particles morphology is frozen rapidly upon fast evaporation, thus preventing damaged surface states.
All swabs were frozen (−20°C) upon collection from healthy individuals.
This unexpected lack of Bacteroidetes has also been recorded in other studies, where fecal samples have been frozen immediately upon collection [13], [17], [43].
Cell lysates were frozen immediately upon collection.
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CEO of Professional Science Editing for Scientists @ prosciediting.com