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We consider that another relevant aspect in CC determination is the fact that ligands must be "free" to interact with the metal ions added during the ASV-monitored titration.
Furthermore, I am worried whether the two amino acids at opposite ends of the complementary strands would be free to interact or not.
Neuromelanin granules are probably not membrane-bound, and toxicants in unbound neuromelanin would be free to interact with cellular constituents [ 41].
Also, this model would suggest that the second MutL would be free to interact with an adjacent MutS dimer on DNA.
Once trichomonad CPs digest the proteinaceous cover on the T. vaginalis surface, by any of the two proposed pathways, the adhesins will then be free to interact with the host cell receptors for attachment [ 6, 41, 42].
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These interactions do not involve PDZ domains, which are free to interact with additional protein partners 24.
For example, it is possible that the exofacial thiol targets of parthenolide are reduced on cancer cells, and thus are free to interact with the drug, but are oxidized on normal cells, thus unavailable for interaction with parthenolide.
How the PRH TLE interaction is regulated in cells and under what circumstances PRH is free to interact with other partners, including activators and co-activators, is yet to be explored and research in these areas will throw light on context-dependent functions.
Although miR-122 is highly abundant, it is unknown how much bioavailable miR-122 within hepatocytes is free to interact with HCV.
In the absence of ligand, peptides expressed as fusions in an FKBP-peptide-FRB-GST LiRP scaffold protein are free to interact with target proteins.
The simplified model problem includes a steady base jet flow, maintained in the absence of disturbances, superimposed with instability waves that are free to interact nonlinearly.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com