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Due to the long flexible linker between PYR1 and ΔNABI1 (> 120 Å; Arai et al., 2004; Figure 1A), ΔNABI1 might be free for substrate access in the ABA unbound conformation.
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Normally the major substrate for heart metabolism is free fatty acids that account for 60 80%.
The other end of the substrate was free to move.
So much for being free.
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From Figure 1, it is apparent that the number of surface nucleation sites promoting catalyst-free growth is large for substrates annealed at 1,000°C and below.
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