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For both protocols, the RNA needs to be fragmented to sizes of 200 - 500 nt.
Efficient hybridization of bacterial DNA requires that long genomic DNA molecules be fragmented to shorter lengths for optimal hybridization.
Large amplicons are optimal neither for fast PCR nor for the subsequent APEX assay, which requires amplicons to be fragmented to ~50 100 base lengths [Fig. 1b].
The extent to which IFP can be fragmented to create bimolecular fluorescence complementation assays has not been explored using laboratory evolution, although fragmented IFP have been discovered through rational design that require assistance from a protein protein interaction for fragment complementation.
In this scheme, long MSDUs should be fragmented to a series of smaller units to be transmitted sequentially one by one and acknowledged as well.
(591) Although sphingolipids are complex, many are relatively easily ionized and can be fragmented to ions that allow the sphingoid base and amide-linked fatty acid to be determined; therefore, stable-isotope labeled precursors (such as U C-palmitate) can be U C-palmitatew biosynthesis of the sphingoid base bacanone as well as N-acylation.
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Subsequently, mRNA was fragmented to short fragments to be used as templates for random hexamer-primed synthesis of first-strand cDNA by fragmentation buffer.
Pooled DNA was fragmented, to produce DNA fragments of uniform small size (ca. 20 ~200 bp).
The purified lipase-positive fosmids were fragmented to 600 900-bp fragments by nebulization, undertaken according to Roche recommendations.
sscDNA samples are fragmented to provide sscDNA fragments of ~50 200 bp, end labeled with biotin and used for microarray hybridization.
Following purification, mRNA was fragmented to ~200 base pair fragments and cDNA generated with ligated adaptors.
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