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If QSOX1A had been cleaved at the cytosolic side of the membrane or within the TM domain, then the cleavage product should be fractionated with the cytosol.
Even though this investigation supports the traditional medicinal usage in the Ethiopian community and the medicinal uses of these plants in different regions of Ethiopia; to obtain the most promising antimycobacterial activity and to conclude their anti-TB activity, these plants should be fractionated with less polar solvents.
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To separate nuclear and cytoplasmic fractions, cells were fractionated with NE-PER NuCytoplasmicytoplasmic Extraction Reagent (Pierce Biotechnology, Rockford, IL, USA).
The gradient was fractionated with a density gradient fractionator (Foxy Jr. Fraction Collector, ISCO), whilst monitoring absorbance at 254 nm using a UA-6 UV detector (ISCO).
The samples were fractionated with Ciphergen's serum fractionation kit with anion exchange resin in 96-well format into 6 fractions.
In this paper, a commercial impact-resistant polypropylene alloy sample was fractionated with preparative temperature rising elution fractionation (t.r.e.f).f
Serum was fractionated with ammonium sulfate, and the fraction precipitating between 50 and 80% of saturation was collected by centrifugation, and dialyzed.
Dried powder of S. cordata whole plant was extracted with methanol and the resultant (SCME) obtained was fractionated with escalating polarity to obtain n-hexane fraction (SCHE), ethyl acetate fraction (SCEE), n-butanol fraction (SCBE) and the remaining soluble portion as aqueous fraction (SCAE).
To increase the coverage of proteins in SELDI protein profiles, the blood samples were fractionated with HyperD Q (anion ion exchange) into six fractions.
The sample was fractionated with reversed-phase high performance liquid chromatography (RP-HPLC) collecting thirty fractions at two-minute intervals.
Then, the peptides were fractionated with ultrafiltration membranes.
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