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This manuscript provides proof of concept that candidate CSC can be fractionated from MPE primary cultures.
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Gradients were fractionated from the bottom (1 ml fractions) and analyzed for PABP, B5 or ICP4 by Western blotting.
After centrifugation for 18 h at 100,000 g, gradients were fractionated from the top into five fractions of equal volume (with the solubilized pellet as the bottom fraction) and analyzed by SDS-PAGE, using a Tris-Tricine buffer system [65] and phosphorimaging using a Storm 840 phosphorimager and allied software (GE Healthcare Life Sciences, Piscataway, NJ).
At the end of the run, the gradient was fractionated from the top into 250 μl fractions.
The gradients were fractionated from the top manually by pipetting into ∼1 ml fractions.
Four components (P1 P4) with different molecular size were fractionated from the conjugate.
Titanium, Nb, Zr, La, Ce, and Nd remain immobile, so the LILE and Pb are fractionated from the HFSE and REE.
To accomplish this goal, IgG was fractionated from sera collected from weanling beagles and regressor beagles and tested for conferring protection by passive immunization.
A series of alkanes (C14 C28), phenolic compounds and aromatic nitrogen species were fractionated from H-tar by SGCC and analyzed by gas chromatography mass spectrometry (GC MS).
After partial deactivation of the conversion of methyl acetate and formaldehyde to methyl acrylate with a fixed-bed reactor, the deactivated catalysts were fractionated from the reactor.
Semicrystalline and amorphous poly(lactic acid) (l-PLA and d,l-PLA, respectively) were fractionated from chloroform solutions using compressed CO2 as an antisolvent.
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