Following analysis of the E. coli ribosome, a significantly more complex mixture, an S. cerevisiae whole cell lysate, was fractionated based on molecular weight and the resulting fractions were analyzed by LC MS/MS to demonstrate the utility of UVPD for high-throughput characterization.
DNA samples were fractionated based on DNA methylation density as previously described [27].
GFP+ cells were fractionated based on expression of CD41, c-Kit, CD34, or CD45 by flow cytometry (Fig. 4A) and were transplanted into lethally irradiated mice with rescue cells (Table S2).
When MSC-CM was fractionated based on heparin binding affinity, a known ligand for all chemokines, only the heparin-bound eluent reversed FHF indicating that the active components of MSC-CM reside in this fraction.
A portion of this RNA was saved for direct conversion into cDNA while the rest was fractionated based on poly(A) tail length into short (≈25 nt) and long (>25 nt) poly(A) tail fractions using the PolyATtract mRNA Isolation System IV (Promega, Fitchburg, WI) as described (Meijer et al., 2007).
Microarray analysis was done on the total mRNA pool as well as on mRNAs that had been fractionated based upon ribosome occupancy.
Translational regulation can be studied on a global scale by interrogating microarrays with mRNAs that have been fractionated based upon ribosome occupancy [ 26].
In the present study, the three main compositions of WS biomass (cellulose, hemicellulose, and lignin) were effectively fractionated based on SE followed by ethanol extraction.
There seems to be no difference in the susceptibility of the high versus the low population to anti-IL-10 reversal, so one would assume that their data should not differ from the published data using Tregs that are not fractionated based on CD44 expression.
The parent compound and metabolites were separated by HPLC and fractionated based on the UV signals from the added unlabeled compounds.
