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Linker sequences are hypervariable: only similarities between MocR linkers from organisms related at the level of species or genus could be found with sequence searches.
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Sequences matching the mature miRNA sequence of two conserved families (miR1511 and miR858) that do not currently have Populus mirBase entries were found with sequence counts of 36 and 17 respectively.
Moreover, no relevant similarity was found with sequences from pathogens or other organisms.
Finally, 833 pairs are found with sequences that differ only at a single nucleotide position.
Rather, bestfits for each LiSIDER2-32 sequence were found with sequences distributed among the different L. braziliensis chromosomes, indicating that SIDER2s are not chromosome specific for all Leishmania species.
BLAST parameters and the accession numbers corresponding to analyzed sequences can be found with coding sequence alignment results in Table S1.
Furthermore, within this study, MAP2325 could be found with 100% sequence identity also in MAP-S strains JIII-386 (Type III) from Germany as well as in CLIJ361 (Type I) from Australia, and confirmed in all MAP-C isolates.
From the 17 unidentified open reading frames in the mitochondrial genome of Euplotes minuta two could be found with significant sequence similarity to T. pyriformis and P. aurelia (Table 2).
This observation is at the basis of building by comparison, one of the most successful methods for computing the 3D structure of a protein sequence when a template is found with a sequence similarity search against the Protein Data Bank (PDB) (11).
Only 17 human KRAB-ZNF genes were found with fingerprint sequence conserved between eutherians and opossum.
A homozygous splice site mutation in the SLC12A3 gene was found with the sequence analysis of the NCCT gene.
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