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Affected individuals should be followed using appropriate standardized instruments as described above.
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A standard horseradish peroxidase staining procedure was followed using an appropriate biotinylated secondary antibody (Vector laboratories, Peterborough, UK) at 5 10 μg ml−1, followed by the elite ABC kit (Avidin : Biotinylated enzyme Complex; Vector laboratories) and DAB (3, 3′diaminobenzidine) as the chromogen substrate (Vector laboratories).
(36) Titrations were followed using additions of the appropriate peptide solution until a minimum of 2.0 equiv of peptide per S100B subunit had been added.
The appropriate procedures were followed when using the equipment to attain high accuracy of speed measurements.
Strict adherence to manufacturer's protocol was followed including appropriate use of control samples.
Two-way ANOVA with fixed effects for drug factor (df = 19), time factor (df = 3) and interaction (df = 57) was followed by appropriate correction for multiple testing (using the Bonferroni or Benjamini-Hochberg procedures).
Comparison between the three modes was followed, when appropriate, by a pairwise comparison by using the Dunn post/hoc test.
Insertion of loxP sites in the chromosome was followed by selection using the appropriate antibiotic and confirmed by PCR analyses using primers SPI19_OUT_UP and SG1021_VEX_H2_U2 for the upstream loxP insertion, and primers SPI19_OUT_DOWN and SG1056_VEX_H2_D2 for the downstream loxP insertion (Table 2).
The CO2 reduction in using lightweight aggregate in concrete is calculated, and is followed by appropriate conclusions and recommendations.
Washing to remove unbound virus was followed by detection using the appropriate primary and labeled secondary antibody.
STROBE guidelines were followed as appropriate.
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