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Yolk protein trafficking can be followed using a yolk protein::green fluorescent protein fusion (YP170 GFP) which has been used to identify genes required for yolk uptake and trafficking such as rme-2 [6].
Fusion of autophagosomes with lysosomes can be followed using a tandem RFP-GFP-LC3 probe.
Individual cargo at the surface could be followed using a reducible fluorescent ligand for the SNAP tag.
Prokaryotic hyaluronidases had become well described because their mechanism of action is an elimination reaction that can easily be followed using a spectrophotometer.
The changing spectral features of interest can then be followed using a suitable mathematical model of light interaction with the tissue and chromophores of interest.
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Contact nucleation and the growth rate of sucrose crystals were followed using a photo-microscopic technique.
Dynamic uptake of tetramethylrhodamine was followed using a fluorescence microscope to estimate small analyte diffusivities into the hydrogel networks.
ASTM methods were followed using a Siwek 20-L explosion chamber, MIKE 3 apparatus and BAM oven.
Endocytosis of the proteoglycan NG2 was followed using a mouse-anti-NG2.EC antibody against the extracellular domain of NG2 (courtesy of Dr. Stallcup).
Four-point bending experimental fatigue tests are performed, where the evolution of the damage is followed using a real-time detection method.
The degradation processes were followed using a range of characterisation techniques including tensile testing, GPC, MAS-NMR, FTIR, WAXS and SAXS.
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