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These, however could be folded manually.
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The filtered pre-miRNA sequences were folded again using MFOLD and checked manually [ 23].
ITS2 secondary structures of the three newly obtained sequences were folded with the help of RNAstructure [ 38] and afterwards manually corrected.
To facilitate carrier operations, the outer wing panels and the tailfin could be manually folded.
Some tRNAs were readily identified by all methods, whereas some proved harder to identify and had to be manually folded in some mitogenomes (e.g., tRNA-S1).
Each Pr77-hallmark in a mobile element repeat was manually folded into an HDV-like ribozyme structure using predicted L1 TcRz and ingiRz foldings as guides.
The folding endurance was measured manually by repeatedly folding a small strip of the film (2 × 2 cm) at the same place until it broke.
Based on minimum free energy ITS2 secondary structures were directly folded with the help of the "RNAstructure" software [ 60, 61] and manually corrected.
The five-speed can be shifted manually.
Each ballot will be inspected manually.
COOL TECHNOLOGY -- Some Magnums' automatic transmissions can be shifted manually.
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