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In this case, the NCs that migrated within a micellar zone can be focused using a preconcentration mechanism.
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The laser beam passed a polarizer and a half waveplate, and was focused using a high numerical aperture objective lens (NA = 0.9, Nikon).
The ejected electrons are focused using a unipolar electrostatic lens and conical electrostatic mirror to form a magnified image on a microchannel plate (MCP).
A linearly polarized irradiation laser beam of 1,030-nm 1,030-nmth wavelengthd using a concave lens of 12.5-mm focal length.
Incident X-rays ((lambda =) 0.9744 Å, 12,735 eV) were focused using a bent cube root I-beam Si (311) monochromator.
The excitation line was focused using a 63x (NA = 0.9) water immersion objective for an integration time of 15 s using an inverted microscope system.
The laser beam is focused using a lens of focal length 185 mm and the transmittance is measured using a pyroelectric energy probe as a function of sample position z by translating the sample along the beam axis (z-axis).
Excitation light was focused using a 60× water-immersion objective (CFI Fluor; 1.0 numerical aperture; Nikon).
The beam was focused using a 63× objective (Nikon, Japan).
Proteins were focused using a Protean IEF Cell (Bio-Rad) at 12°C as described [ 83].
For these measurements the laser beam is focused using a 20x objective on either a BBO crystal to generate SHG signal or on a 1 mm thick glass slide to generate THG.
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