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We recommend that distal fragments should be fixed with at least seven cortices for this type of osteotomy.
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The fractures were fixed with at least two lateral or cross Kirschner wires (K-wires) under fluoroscopy control due to fracture pattern and stability and surgeon's preference.
Bone segments proximal and distal to the corticotomy site were fixed with at least 3 pins.
The graft was fixed with at least two screws on the superolateral acetabular defect so that it could cover especially the posterior superior portion of the defect.
"You have patients with other problems that need to be fixed" with surgery at the same time, he says.
For confocal microscopy, McCoy cells infected with Chlamydia and incubated at 28°C were fixed with methanol at 48 and 120 h p.i., washed in PBST, and stained with antibodies as described above.
Cells grown on coverslips were fixed with methanol at −20 °C for 6 min or 4% paraformaldehyde/phosphate-buffered saline at room temperature for 15 min.
Plate was fixed with screw at longitudinal dynamic hole.
The acellular tissues then were fixed with genipin at various known concentrations to obtain varying degrees of crosslinking.
Cells were fixed with methanol at −20°C for 5 min.
The resulting cell pellet was fixed with acetone at -20°C for 30 min.
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