Honeybees were fixed individually on a metallic holder so that they built a bridge between two brass plates through which a 2 sec mild electric shock (7.5 V) was delivered by a stimulator (60 Hz-AC current) (Fig. 1).
Salt concentration was set at 0.2 M. Each MD simulation run was then continued for at least 20 ns at the modeling conditions of pH 4.5, 5.6, 6.0, 6.18 6.28, 6.5, 6.5, 7.0, 7.4 and one of the His residues (His251, His284 or His297) was fixed in protonation state individually at pH 5.6 and 7.0.
For evolutionary dynamics this means that the σ 's are fixed for every column individually, the neutral mutation process defined by the μ 's is shared between the columns, and the sequences have diverged beyond the relevant correlation times and can thus be considered independent.
Individually harvested organs were fixed in 10% neutral buffered formalin and whole specimens were placed in several volumes of 10% neutral buffered formalin.
Nevertheless, these materials cannot be used for certain learning activities, such as learners observing program behavior where input data are changed individually, because the input data are fixed.
Fetuses were individually weighed, and two-thirds of them were fixed in Bouin's solution and examined for soft-tissue anomalies.
If the predefined condition was not met, different sets of parameters, individually and in combination with each other, were fixed and the model reassessed.
Similarly, Raman spectra were acquired from individually dispersed cells drop cast onto a glass slide and was fixed with 4% paraformaldehyde.
The sections were fixed 30 s with 70-75% ethanol (Fluka, Ref: 02855, Sigma-Aldrich) and stained individually at room temperature before LCM.
Glasser's testimony took three hours, as he was cross-examined individually about every single case that the government had alleged to have been fixed.
