Sentence examples for be fixed in primary from inspiring English sources

The progressive nature of the insurance scheme could be fixed in primary legislation, but the details subject to political decision-making by serving Health Secretaries.

After complete relaxation, specimens were fixed in the primary fixative or in 1% glutaraldehyde buffered in phosphate buffer (1.28 mM NaH2PO4 plus 5.38 mM Na2HPO4, pH 7.4).

Prior to SEM imaging, the cell culture was fixed in mixed primary fixative of 2% paraformaldehyde/Krebs/sucrose and 2.5% glutaraldehyde in phosphate buffer at pH 7.4 for 30 min, then rinsed three times with phosphate buffered saline (PBS) solution.

Cells were fixed in methanol and primary antibody to p-ERK (Santa Cruz Biotechnology, Santa Cruz, CA, USA) applied in a dilution of 1 50 followed by TRITC-conjugated secondary antibody (Dako, Ely, UK) (1 200).

The immunoprecipitates and whole cell lysates were subjected to SDS-PAGE, transferred to Immobilon-P membranes and the association of RPTOR with MTOR determined using western blot techniques as described above., Freshly isolated lenses, lenses from organ culture, and primary lens cell cultures were fixed in 3.7% formaldehyde (15 min for primary cultures and overnight for whole lenses).

For other stains, brain sections are fixed in acetone and stained using primary antibodies for CD34 (1 200 dilution; DAKO).

For cellular immunostaining, primary keratinocytes were fixed in 3% formaldehyde or in 100% methanol, followed by incubation with primary and secondary antibodies (see Supplemental Experimental Procedures).

Tissue was fixed in a cacodylate-buffered glutaraldehyde-paraformaldehyde primary fixative, osmicated and embedded in Epon.

The primary AECs or lung specimens were fixed in 4% paraformaldehyde, stained with the appropriate primary antibodies and incubated with FITC-conjugated anti-mouse IgG or Cy3-conjugated anti-rabbit IgG (Jackson ImmunoResearch, West Grove, PA, USA).

The rat brain tissues or mouse primary neurons were fixed in buffered 4% (v/v) formaldehyde for 5 min at room temperature (RT).

Blocks from the primary tumour were fixed in 10% buffered formalin and embedded in paraffin wax.