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For ODN containing three consecutive heterologies, one should use A long ODN, at least 10 times longer than the number of heterologies, otherwise, the presence of "bistable states" in the pairing of the two molecules will be extremely unfavourable to the subsequent steps of recombination.
In general, we recommend using ODN at least 10 times longer than the size of the correcting sequence; otherwise the presence of "bistable states" in the pairing of the two molecules will be extremely unfavourable to the subsequent steps of recombination.
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The presence of those bistable states might be extremely unfavourable for the next steps of homologous recombination i.e. the strand exchange.
The present case confirms that the outcome of erlotinib related ILD may be extremely unfavourable[ 1].
The political situation was extremely unfavourable, however, since Judah south of Hebron had been occupied by Edomites escaping from Arab pressure, while the tiny remainder north of Hebron had passed under the control of the governor of Samaria.
Attitudes towards Aboriginal people were self-reported on an attitude thermometer which provided 10° intervals ranging from 0° (extremely unfavourable) to 100° (extremely favourable), with the mid-point of 50° representing neither favourable nor unfavourable attitudes.
This confirms the idea that, when three consecutive heterologies are placed near the centre of an ODN45, they can be crossed and lead to a stable triplex, whereas ODN25 containing three heterologies form an instable triplex with "bistable states" extremely unfavourable to the following steps of homologous recombination.
Be extremely faithful to it.
It's important to be extremely gentle.
I'm extremely happy to be here.
However, Nissan is thought to believe these options would be unfavourable to its existing shareholders and is therefore looking at other options.
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CEO of Professional Science Editing for Scientists @ prosciediting.com