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Study data will be extracted using standard forms and entered into Excel spreadsheets in tabular form.
The 10-year probability of CHD will be assessed and calculated at the first study visit based on Wilson et al. [ 7].> Twenty mL of blood will be drawn and sent to a CLIA-certified laboratory and DNA will be extracted using standard procedures.
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DNA was extracted using standard extraction kits following the manufacturer's protocol (Blood and Tissue DNA extraction, Qiagen).
For genotyping by Southern blot analysis, DNA from ES cells or tissues was extracted using standard DNA extraction procedure.
Total RNA was extracted using standard Trizol extraction (Invitrogen, Carlsbad, California).
The medical records of identified patients were reviewed and data were extracted using standard data extraction form.
A single leg was removed from dried or ethanol-fixed specimens and DNA was extracted using standard glass fibre extraction protocol [10].
Genomic DNA was extracted using standard phenol-chloroform extraction.
Placenta genomic DNA was extracted using standard phenol-chloroform extraction methods.
All the samples were extracted using standard metabolic solvent extraction methods and analyzed through GC/MS and LS/MS as previously described [ 44].
Total genomic DNA was extracted using standard 3-step phenol/chloroform extraction methods [ 37].
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