Dried leaf material (200 mg) from three individuals of each population was extracted in separate vials containing 10 ml diethyl ether for 24 h as described in the literature (Fattahi et al. 2013; Vieira et al. 2001).
It is therefore tempting to conclude that the different cues are extracted in separate regions in the vicinity of the posterior TE region, from which Yamane et al. [4] recorded, and then converge upon it.
For this reason the state variables encoding spatial information will be extracted in a separate set denoted as spatial state variables (SpSV).
No additional items were extracted for the three-group comparison than had been extracted in both separate 22q11DS-ASD and KS-ASD versus heterogeneous ASD comparisons.
The study results [mean and standard deviation (SD)] for total T, free T and SHBG concentrations in International System (SI) units were extracted in a separate standardized form.
After pre-treatment with 5-Aza-dC or Stat3 inhibitor, rhOSM was treated in serum-free condition for 30 min, and whole cell lysates were extracted in RIPA buffer, separated on 4 12% gradient SDS-PAGE, and transferred to nitrocellulose.
Liver tissue proteins were extracted in RIPA buffer, separated using SDS-PAGE, transferred to polyvinylidene fluoride (PVDF) membrane, and western blotted for different proteins using specific antibodies.
Sediment samples were lyophilised and homogenised, after which pigments were extracted in 90% acetone, separated using reverse-phase HPLC, and measured with a Gilson fluorescence detector according to Wright & Jeffrey [37].
Lipids of late endosomes and late endosomes fractions obtained with OptiPrep gradient centrifugation were extracted in CHCl3/MeOH and then separated by two-dimensional thin layer chromatography (TLC) for phospholipids analysis [25], [44].
Total protein from cultured cells or mouse tissues was extracted in lysis buffer and separated using SDS-PAGE.
