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Because adipose tissue is present in such a high abundance within the human body, the number of extractable Muse-AT cells is abundant compared to that which can be extracted from bone marrow or dermis.
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Such finds are incredibly important to palaeontology as inevitably there is a limit to what information can be extracted from bones alone.
Part of the problem was that ancient DNA is fragmentary, and most of it breaks down into single strands after it is extracted from bone.
aDNA was extracted from bone sample G483 (Denmark) and amplified by to confirm result.
DNA was extracted from bone powder using GENECLEAN Kit® For Ancient DNA (Q-BIO gene) according to the manufacturer's instruction.
DNA was extracted from bone in the same way, except for an initial decalcification of the bone shavings (∼10 50 mg) by incubation at room temperature, with rotation, overnight in ∼500 ul of 0.5 M EDTA pH 8.0.
DNA was extracted from bone marrow samples of ALL, AML, CML, CLL, and MM at diagnosis, diagnostic tissues (either lymph node or nasal biopsy in nasal NK-cell lymphoma) in patients with NHL, and lymphoma and MM cell lines by standard method.
RNA was extracted from bone using TRIzol Reagent (Invitrogen, USA).
For the E. canis n-PCR assay, DNA was extracted from bone marrow aspirate.
Total RNA was extracted from bone and osteoblasts, and gene expression was profiled by quantitative reverse transcription polymerase chain reaction.
Genomic DNA was extracted from bone marrow in patients with acute myeloblastic leukemia (AML) and peripheral blood in healthy donors.
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