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After homologous recombination, gfp should be expressed under control of the SPIG1 gene regulatory unit.
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To secure high expression, the construct was expressed under control of the maize Ubiquitin-2 constitutive promoter (2 kb) [ 55].
The cDNA of C. neoformans SSN8 gene was subcloned into pYES2 yeast expression vector to generate pYES2 CnSSN8, which SSN8 was expressed under control of the GAL1 promoter.
These selected genes were expressed under control of the Cauliflower Mosaic Virus 35S promoter, which enabled their expression to high levels.
Smc1α was expressed under control of the Smc1β promoter in either wild-type or SMC1β-deficient mice.
The thermostable BgaB was compared to the commonly used LacZ β-galactosidase from E. coli, which was expressed under control of the same three promoters.
In these assays, the effector gene, OsSMF1, was expressed under control of the P35S promoter and co-transfected together with the reporter construct into the protoplast.
In transgenic mice in which the green-fluorescent protein is expressed under control of the Rag2 promoter ("Rag2-GFP mice"), newly developing T cells are GFP+ and cells that are "older" do no longer express GFP (Yu et al., 1999).
The recombinant plasmid (poabf62a-pPIC9K) was therefore transformed into GS115 cells by electroporation, and recombinant PoAbf62A was expressed under control of the alcohol oxidase promotor in GS115 and purified from the culture medium by ammonium sulfate precipitation and column chromatography.
In both strains 2-methylcitrate synthase was expressed under control of the constitutive kanamycin-resistance gene (ΩKm) resulting in up to 20-fold higher specific 2-methylcitrate synthase activities in comparison to the wild type.
The constructs were transformed in to E. coli strain ER2566 and the transcripts were expressed under control of IPTG.
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