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The unmodified protein can be expressed to levels easily detectable by epifluorescence microscopy without adverse affect on the viability of plant cells.
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Sorting the cells for low GFP levels yielded populations where the p130Cas variants were expressed to levels similar to endogenous p130Cas in Cas +/+ MEFs (Fig. 1B).
E7GGG was expressed to levels of 0.12%% of total soluble protein (TSP).
We have designed a hexahistidine tagged version of a modified diphtheria toxin B-fragment (DT-BHis) that can be expressed to high levels in E. coli.
Vrn1 must be expressed to sufficient levels for VS to occur.
(24) The resulting protein GB1-UbldV56E could be expressed to high levels and purified to homogeneity for structural characterization.
The V. proteolyticus 5S rRNA has previously been shown to be expressed to significant levels and incorporated into ribosomes without significant detriment to growth [ 22].
CEA is expressed to high levels on tumour, yet is also expressed to far lower levels on normal gastric mucosal surfaces (Hammarstrom, 1999; Francis et al, 2002).
The resulting mutants were expressed to high levels in Escherichia coli.
The recombinant dengue multiepitope protein was expressed to high levels in Escherichia coli, purified in a single step, yielding >25 mg pure protein per liter culture.
Each of the RB proteins was expressed to similar levels (Fig. 1D, lower panel).
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