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Do the individual hallmarks need to be expressed simultaneously or sequentially along the continuum of carcinogenesis (from exposure to unambiguous cancer phenotype development)?
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Sometimes, MMP1 is expressed simultaneously or before ETS1 and JUN.
Intermediate filaments from two different families can be expressed simultaneously, however, in tumors or in development [ 37, 38].
At least three different exogenous genes could be expressed simultaneously and stably over multiple passages.
Even when restricted laser sources are employed, a small number of color codes can be expressed simultaneously.
By following this latter perspective, a model for coalition structure generation is proposed where constraints of two different kinds can be expressed simultaneously.
However, for the development of a durable RNAi-based gene therapy against HIV-1, multiple shRNAs need to be expressed simultaneously in order to avoid viral escape.
Alternatively, a 2A sequence, which allows for multiple genes to be expressed simultaneously under the same promoter, could be used (Kim et al. 2011; Shah et al. 2015; Geier et al. 2015).
To fully exploit the advantages of bacterial luciferase, all five genes (luxCDABE) of the lux operon must be expressed simultaneously.
This may imply that in C. carboxidivorans, the activity of the butyryl-CoA dehydrogenase requires the electron transfer flavoprotein and that the genes encoding those proteins should be expressed simultaneously, as has been previously shown in C. acetobutylicum [33], [39].
These data suggest that information about social group membership may be expressed simultaneously from multiple cues for use by both explicit and implicit perceptual processes an insight that likely may not have been gained from studying groups for whom perception is obvious because the task of judging group membership from individual features might be too easy.
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