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eGFP, the sensor for PR activity, will be expressed only when PR/Gal4 expression is induced in the presence of a PI.
Another targeting vector, pcDNA5/FRT, has an FRT sequence fused to a promoterless hygromycin-resistance gene that can be expressed only when correct gene targeting occurs.
Specifically, we propose that ER will be expressed only when estrogen is present in the microenvironment.
To further confirm that the IRE1-dependent splicing of xbp1 mRNA was induced by HTorAΔE, we used a xbp1-eGFP reporter that allowed eGFP to be expressed only when a 23 bp intron was spliced out of the xbp1-eGFP construct by IRE1 in the ER [ 31].
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Normalized intensities were used as gene expression levels and genes were defined as being expressed only when their expression was higher than 99% quantile value of the expression of all negative control spots across all the arrays in this study (Figure 1a) as described by Zhang et al. [17].
This is achieved by the sequential location of these regulator genes on the chromosome and through the use of DNA methylation to control gene expression; for example, the dnaA promoter is expressed only when DNA is fully methylated while hemimethylation promotes transcription of ctrA [17].
Trypanosome chromosome-internal VSG genes are expressed only when they are duplicated into subtelomeric expression locations [ 47, 48].
Alternatively, the gene encoding the PduO ACAT is expressed only when 1,2-propanediol is present, whereas expression of the gene encoding EutT requires the presence of ethanolamine and AdoCbl.
We designed a system in which GFP protein is expressed only when exon 7 of is included in alternative splicing.
Increased preference for a morphine-paired environment is expressed only when drug-cue conditioning occurs during the abstinent state.
Chlorophyll is synthesized in an environment containing light because the gene for chlorophyll is expressed only when it interacts with light.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com