In addition, our results shown that retaining the 6 N-linked oligosaccharides within a short B-domain spacer associated with 2A and furin cleavage site is expressed more efficiently both in vitro in traditional heterologous expression systems as well as in vivo in a mouse model of hemophilia A.
We found that PTPN22.1 and Lyp2 were expressed more efficiently than PTPN22.2, PTPN22.5, PTPN22.6, and PTPN22.8.
However, we identified several other cbh1 genes that were expressed more efficiently: the activity of T.e.CBH1 and its derivative with the T.r.CBM attached to its C-terminus exceeded that of T.r.CBH1 by at least two orders of magnitude, yielding 100-200 mg/L in shake flasks and 300 mg/L in high cell density conditions.
Both Pre-mix and Post-mix delivered CpG-DNA to DC endosomes, where TLR9 is expressing, more efficiently than free CpG-DNA solution did.
Moreover, constructs with the CD28 signaling domain proximal and the ζ -chain distal to the membrane were found to express more efficiently in Jurkat than constructs with the opposite orientation (Finney et al., 1998), thus determining the signaling element arranging pattern adopted by other researchers in the years since.
They concluded that adenoviral vectors are able to express more efficiently than retroviral vectors in ACL cells and can lead to a considerably long period of gene expression in vivo (six weeks).
The transformant strain in which the truncated pfk13 gene of A. niger was expressed performed more efficiently as it was able to grow successfully in glucostat cultures with 277 mM glucose – while the optimum glucose concentration for the parental strain was 55 mM.
A three-partite fusion protein (consisting of NP, M1 and NS1) was expressed much more efficiently than the four-partite protein.
The design of the mutants was based on the DrAqp3b deduced amino acid sequence since the corresponding cRNA was expressed apparently more efficiently in X. laevis oocytes than that of DrAqp3a.
However, this difference may be simply a matter both of NELF-B protein being expressed ∼9-fold more efficiently in 293 cells and of using more NELF-B plasmid in 293 cells, thereby making the curvature more evident.
