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In all cases, both NDUFS2 alleles were found to be expressed (data not shown).
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Interestingly, in SC4 cells, we did not observe a significant increase in either periaxin expression or downregulation of c-Jun when KROX20 was expressed (data not shown).
We sequenced the cDNA clone yk1713e02 to confirm that residues 216 231 are expressed (data not shown).
T1 lines containing the Nbs2-Rdg2a transgene were fully susceptible to both the leaf stripe isolates (Table 1), although RT-PCR confirmed the transgene was expressed (data not shown).
In our study, microarray analysis indicated that the metQ2 gene is expressed (data not shown).
Indeed, in cultured HRMCs, Nox2, Nox4, and Nox5 were expressed (data not shown).
However, in cultured HRMCs, we only observed that Nox2, Nox4, and Nox5 were expressed (data not shown).
The mRNA sequencing data for Ae t. arabicum supported the evidence that all 67 putative AabGS genes were expressed (data not shown).
This was confirmed by immunostaining for Notch protein which indicated no changes in Notch protein levels in the wing disc where CLN3 was expressed (data not shown).
At the protein level, we failed to detect changes in TGF-β1 and TGF-β3, although both isoforms were expressed (data not shown).
Moreover, immunoblotting of whole-cell lysates of this cell line demonstrated that Ptena protein is expressed (data not shown), supporting the notion that the tumors in ptena +/−ptenb −/− fish are not caused by loss of heterozygosity.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com