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The table shows mRNA transcripts encoding for cytokines as found to be expressed by microarray in Chlamydia trachomatis-infected human monocytes over a time course of up to 7 days.
The table shows mRNA transcripts encoding for chemokines as found to be expressed by microarray in Chlamydia trachomatis-infected human monocytes over a time course of up to 7 days.
The table shows mRNA transcripts encoding for cytokine and chemokine receptors as found to be expressed by microarray in Chlamydia trachomatis-infected human monocytes over a time course of up to 7 days.
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At the RNA level, 189 genes were detected to be differentially expressed by microarray technology; and at the protein level, 16 proteins were detected to be differentially expressed by 2D-DIGE.
Interestingly, only a small subset of genes was found to be differentially expressed by microarray analysis and many of these have been implicated in controlling apoptotic pathways (i.e. Erdr1, Ier3, Pdap1, Calm2, Hspca, Mtch1, and Ifitm1 and 2; Table 1).
Selected genes found to be differentially expressed by microarray were validated with qRT-PCR as previously described [ 26].
Nearly all of the genes previously identified as members of the C. crescentus Fur regulon [ 6] were found to be differentially expressed by microarray analyses, validating the experimental procedure.
In addition, expression of several genes thought to be potential candidate genes, based on their roles in carcinogenesis but not found to be differentially expressed by microarray analysis, were measured by qRT-PCR.
It is reassuring that most genes identified in the mutant screens were also found to be differentially expressed by microarray analysis.
Quantitative PCR was performed on 11 genes found to be differentially expressed by microarray hybridization as well as a housekeeping gene used as an internal control (bovine ribosomal protein L19; RPL19).
A group of genes of interest found to be differentially expressed by microarray analysis as well as a few that did not change in expression were further validated by real-time RT-PCR assay.
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