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The main objective of the present study was to determine whether GILZ, an anti-inflammatory protein that we recently found to be expressed by human neutrophils in particular stimulatory conditions in vitro [17], is modulated during ARDS.
Recently, the chemochine CCL5 (RANTES) was found to be expressed by human prostate carcinoma cells and reported to stimulate their proliferation and invasion [10].
All novel marker genes analysed were shown to be expressed by human NP, AF and AC cells.
Such candidate antigens include CD133, CXCR4 and Robo4, all of which have been reported to be expressed by human HSCs.
It is of particular relevance for the present study that all three mesothelin transcript variants have been reported to be expressed by human cancer cells.
Such immunomodulatory and anti-inflammatory function could extend beyond the liver, as LSECtin has also been demonstrated to be expressed by human peripheral blood and thymic dendritic cells and monocyte-derived macrophages (Dominguez-Soto et al. 2007).
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The major finding of this report is that primary cilia are expressed by human astrocytes but this expression is disrupted generally during early ciliogenesis in cultured astrocytoma/glioblastoma cells.
Furthermore, there were also differences in cell-specific gene expression; notably, SNAP 25 and TNMD were expressed by human AC cells and FBLN 1 was expressed by human AF cells at levels very similar to those observed in AC cells.
It has been previously suggested that both COX-1 and COX-2 are expressed by human RA FLS and that the expression of COX-2 messenger RNA and protein is enhanced by proinflammatory cytokines such as IL-1β and tumor necrosis factor α [ 17].
Our suggestion is based on work by Cerutti's group who have clearly shown that functional toll-like receptor (TLR 3 is expressed by human tonsillar B-cells, with higher expression in GCs and sub-epithelial regions, but is absent from memory B cells (CD27+ cells).
Concerning the dependence of GARP expression on FOXP3 as described above, it has been reported that FOXP3 is expressed by human and murine megakaryocytes [ 47].
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