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This miRNA was originally cloned in a murine system and therefore there is no data demonstrating that the human homologue can be expressed and processed in HEK293 cells.
45 46 Emotions need to be expressed and processed over time in order to facilitate adjustment 44 which may require social and professional support.
The in situ hybridization (ISH) revealed the expression of miR-1290 in only transfected cells (Supplementary Figure 3), and qRT-PCR corroborated expression of miR-1290 in transfected cells but not mock-transfected cells (>100-fold, P<0.001, n=3, t-test), confirming its ability to be expressed and processed.
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In vitro study showed that eGFP2A protein, as expected, was expressed and processed properly from the nascent viral polyprotein.
Cell lysates expressing wild type Spike showed the characteristic doublet of SARS spike glycoprotein (upper panel, lane 1), indicating that SARS spike glycoprotein is expressed and processed to incorporate high mannose or complex sugars [33].
quinquefasciatus pre-miRNAs were identified (Table 2), indicating that both hairpins are expressed and processed.
It is expressed and processed predominantly in liver for plasma delivery, and it is excreted by the kidney.
This construct was expressed and processed in DU145 cells in a manner identical to that of wild-type APP695 (Fig. 4B).
Several of the miRNAs from the murine Sfmbt2 locus are expressed and processed in placenta (Additional file 6, Figure S6), not surprising given that they are part of the primary transcript for Sfmbt2, which is robustly expressed in placenta.
Clostridium difficile prophages can contain CRISPR arrays that are expressed and processed (Sebaihia et al. 2006; Soutourina et al. 2013), and spacers match to other phage genome sequences, which could in theory limit genetic exchange (Hargreaves, Flores, et al. 2014).
To test whether artificial short hairpins could be expressed and properly processed to produce a designed guide strand, different short hairpin configurations carrying the same inserted guide strand sequence were cloned in the Rheoswitch expression vector pNEBRX1.
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