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Although functional interactions between Hcrt neurons and these populations have yet to be explored in zebrafish, the anatomical and molecular similarities of zebrafish and mammalian brains suggest that at least a portion of Hcrt-induced arousal is likely to be mediated by these centers.
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Apart from the previously identified FIN neurons6, also ipsilateral and ascending, the role of the vast majority of engrailed1b+ hindbrain neurons has not been explored in zebrafish.
The ability of COS to shape the development and morphological patterning in vertebrates has been explored in zebrafish.
The conservation of TOR signaling pathway has been explored in zebrafish using both pharmacological manipulation and morpholino gene knockdown [67].
Loss-of-function models of neurodegeneration have also been widely explored in zebrafish (see [20] for review).
Apart from in vitro studies, miRNA mutant and transgenic lines should be explored as these lines in zebrafish have widened our understanding of the molecular processes that govern the phenotypic outputs of several transcriptional factors, signaling molecules, and genes.
Although hypoxia-elicited gene expression in zebrafish has been explored by microarray in several previous studies [ 27- 30], RNA-seq characterization may give new information due to its advantages over microarray.
UCP homologs in zebrafish were explored from genomic sequence analysis to transcript expressions.
Methods for large-scale generation of transgenic zebrafish were explored [ 9].
Using whole-mount in situ hybridization, the spatiotemporal expression of the zc4h2 ortholog during zebrafish embryonic development was explored.
The functional significance of caspase activity in inflammation in zebrafish and the implications for IL-1β function have not previously been explored.
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