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The retention of probes is also an important consideration, as over time all molecular probes will be expelled from cells, either by active extrusion or by passive leakage.
Because Hoechst 33342 would be expelled from cells via verapamil sensitive ATP-binding cassette (ABC) transporters, a subset of the cells were incubated with 50 µmol/L verapamil for 30 min at 37°C before adding Hoechst 33342 to block the efflux of fluorescent dye from SP cells within the A549 population.
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However, it is not only the free acid form that is expelled from cells as one multidrug resistance protein (MDR1) has been shown to extrude the AM-ester, but not the hydrolyzed free acid form of sensors, suggesting multiple mechanisms for expulsion of dyes.
It is now clear that exosomes from different malignant cells carry ABC transporters, and some studies have shown that drugs may also be expelled from the cells through exosomal mechanisms [ 41, 81, 85, 86].
In contrast to the expulsion of the symbionts by inclusion in aposomes, the specific exocytosis of the algae implies a specific recognition of the alga as an object to be expelled from the cnidarian cell.
We reasoned that if less cells would be expelled from the epithelial layer, the disruption of multicellular junctions would be reduced, even if LT-paralyzed motility had not been restored.
He hinted it may be expelled from Afghanistan.
The Maldives should also be expelled from the Commonwealth.
Republicans called for Mr Jefferson to be expelled from Congress.
Expulsion: the incumbent will be expelled from the market.
They are likely to be expelled from Melilla.
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CEO of Professional Science Editing for Scientists @ prosciediting.com