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In this review, we discuss what can be expected when imaging mitochondria with conventional diffraction-limited and diffraction-unlimited microscopy.
The homemade phantom acquisitions demonstrated that, on average, a 10.3% overestimation could be expected when imaging a 18F-FDG source that mimics a pertinent-sized tumor surrounded by contrast media, whereas measured activity was almost equal to true activity when the source was surrounded by water.
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What could be expected when combining logics?
As expected when imaging the 'front' of the NPC (i.e., looking at it from the cytoplasm), the signals from the Nup358 and imp-β spatially merged when imaging the basal surface of the nucleus.
What is a level of brightness that can be expected in biological imaging?
250 The UK and French series both included patients from many years ago, when survival rates would be expected to be lower, and many patients in these series predated modern imaging techniques.
Consequently, additional alternative imaging would not be expected of any incremental benefit.
When integrated with high-content imaging of intact cells prior to electrophoresis, the assay throughput falls considerably to ∼0.1 cells/min, as would be expected without the use of a high-speed live-cell imaging system.
Obviously with different imaging parameters and quantification techniques, different results would be expected.
Molecular imaging is another promising research field where improvements may be expected.
Given recent advances in and wider availability of complex imaging, physicians are expected to understand imaging appropriateness.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
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CEO of Professional Science Editing for Scientists @ prosciediting.com