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As apoptosis-resistant human gammadelta-T cells can now readily be expanded to large numbers (clinical scale), these findings must be considered in the context of developing adoptive immunotherapy strategies to exploit gammadelta-T cell innate immune responses for the primary or adjuvant treatment of breast cancer.
Primary MSCs can be expanded to large numbers in vitro, which is a prerequisite for high-throughput RNAi screening techniques.
In addition, they described these cells as showing a limited senescence and able to be expanded to large numbers of cultures.
Mature T lymphocytes are easily harvested from the peripheral blood of donors and can be expanded to large numbers in vitro using cell surface stimulation with antibodies to the markers CD3 and CD28 [ 45].
Mesenchymal stemcells (MSC), with their ability to be expanded to large cell numbers without losing their differentiation capacity into the myogenic lineage, could offer a promising cell source to generate neomuscle tissue.
Deniger et al. have recently proved that T cells with polyclonal γδ TCR repertoires can be expanded to large numbers (>10) using IL-2, IL-21 and clinical grade artificial antigen-presenting cells, engineered to express co-stimulatory molecules CD19, CD64, CD86, CD137L and membrane-bound IL-15.
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A 64-word design has been developed using 19,000 transistors and it could be expanded to larger sizes easily.
Therefore the EEM parameter sets need to be expanded to larger sets of molecules and further improved.
This is particularly evident in the current small number of clinical studies related to kidney disease that could ultimately be expanded to large-scale clinical trials.
More importantly, this proof-of-principle study can be expanded to larger sample sizes to illustrate biological perturbations in kinase activities in a given disease.
This approach could easily be expanded to larger datasets where specific combinations of variables that are particularly beneficial might become apparent.
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