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The parallel implementation is configured to be executed using four threads.
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Using the CIPRES Gateway, two runs of MrBayes were executed using four MCMC chains and 10 generations, sampled every 1,000 generations.
Experiments were executed using three commonly used types of fertiliser (CAN (Ammonium-nitrate fertiliser), NPK (Compound fertiliser), KCl (Potassium-chloride fertiliser)) with different physical properties.
The protocol was executed using three steps.
The pilot-based estimates of the transition probabilities and of h2 are computed using five EM iterations, while the code-aided refining is executed using eight EM iterations.
The study has been executed using three pre-operative and three post-operative subjects.
The analysis was executed using two independent Monte Carlo Markov Chains and the CAT model.
Analysis of methylated and unmethylated CpGs was executed using two online programs: BISMA (http://biochem.jacobs-university.de/BDPC/BISMA) and QUMA (http://quma.cdb.riken.jp).jp
The one-pot process was executed using three non-polar solvents commonly used in one-pot methods: petroleum distillates (camper fuel), heptane/ether mix (automotive starter fluid) and hexanes.
This multi-fiber approach can be executed using as few as two optical fibers with different diameters [ 26]; moreover, such a device can easily be developed to sample both R SF and F SF.
All analyses will be executed using SPSS.
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