The large intestine was excised and separated into cecum and colon at the cecocolic junction.
The hearts were excised and separated into 2 halves along the anterior longitudinal middle line.
The GI tract was excised and separated into six segments: the stomach, the upper 20 cm, middle and lower 20 cm of the small intestine, the caecum and the large intestine.
Proteins were then separated by BN-PAGE, bands corresponding to complex I alone (band I, Fig. 2A and B) and S1 supercomplex (I + III2 + IV, Fig. 2A and B) were excised and subunits were separated in denaturing conditions.
Synovial tissue was excised and cells were separated by incubating the synovial tissue in RPMI containing 1 mg/ml collagenase IV and 0.1 mg/ml DNase with a vigorous shaking at 37°C for 30 min. The supernatant containing the synovial cells was collected and the undigested tissue was re-extracted.
Uterine horns of rats on days 4 6 of pregnancy were excised and uterine horns on day 6 were separated into implantation and inter-implantation sites.
Protein fractions were separated and protein bands were excised and sent for identification by mass spectrometry (LC-ESI-MS/MS).
The enzymatically active CI and inactive CI complexes were excised and each was placed in a separate microcentrifuge tube.
The altered fragment from one of the normal sample from a separate gel was excised and eluted from the gel using Qia quick gel extraction kit (Qiagen, USA).
A low-percentage acrylamide gel was therefore used to separate these forms, which were excised and analysed by tryptic peptide mass fingerprinting.
