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Both presequences can be exchanged separately by XhoI (eqFP611) and BamHI (smGFP4) restriction digestion, respectively.
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A triangle involving individuals A, B and C is recorded when, within the same 20-second interval, packets are exchanged separately between each of the pairs A-B, A-C and B-C.
The 60S and 40S subunits were exchanged separately into reassociation buffer (10 mM MES-KOH [pH 6], 10 mM NH4 acetate, 40 mM K acetate, 8 mM Mg+2 acetate, and 2 mM DTT), concentrated to 6 μM, and stored at −80°C after being flash frozen in liquid nitrogen.
The partially integrated method involves the solution of the fluid and structural equations separately, but information is exchanged at each time step, so that solution from one domain is used as a boundary condition for the other domain.
The models are calculated separately at each time step; however, their results are exchanged between the models, enabling a simultaneous calculation.
Numbers were exchanged.
Insults were exchanged.
Trash-talking was exchanged.
Taunting insults are exchanged.
Sinister notes are exchanged.
High fives were exchanged.
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